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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which ends up in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two added splice variants had been claimed but thorough nucleotide sequence details is just not but out there.
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Blocking peptides are peptides that bind exclusively towards the concentrate on antibody and block antibody binding. These peptide commonly contains the epitope identified with the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs into the really conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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